Multiple driving forces required for efficient secretion of autotransporter virulence proteins. Journal Article uri icon

Overview

abstract

  • Autotransporter (AT) proteins are a broad class of virulence proteins from Gram-negative bacterial pathogens that require their own C-terminal transmembrane domain to translocate their N-terminal passenger across the bacterial outer membrane (OM). But given the unavailability of ATP or a proton gradient across the OM, it is unknown what energy source(s) drives this process. Here we used a combination of computational and experimental approaches to quantitatively compare proposed AT OM translocation mechanisms. We show directly for the first time that when translocation was blocked an AT passenger remained unfolded in the periplasm. We demonstrate that AT secretion is a kinetically controlled, non-equilibrium process coupled to folding of the passenger and propose a model connecting passenger conformation to secretion kinetics. These results reconcile seemingly contradictory reports regarding the importance of passenger folding as a driving force for OM translocation but also reveal that another energy source is required to initiate translocation.

publication date

  • April 1, 2015

Full Author List

  • Drobnak I; Braselmann E; Clark PL

Other Profiles

Additional Document Info

start page

  • 10104

end page

  • 10116

volume

  • 290

issue

  • 16