Flash memory: photochemical imprinting of neuronal action potentials onto a microbial rhodopsin. Journal Article uri icon



  • We developed a technique, "flash memory", to record a photochemical imprint of the activity state--firing or not firing--of a neuron at a user-selected moment in time. The key element is an engineered microbial rhodopsin protein with three states. Two nonfluorescent states, D1 and D2, exist in a voltage-dependent equilibrium. A stable fluorescent state, F, is reached by a photochemical conversion from D2. When exposed to light of a wavelength λ(write), population transfers from D2 to F, at a rate determined by the D1 ⇌ D2 equilibrium. The population of F maintains a record of membrane voltage which persists in the dark. Illumination at a later time at a wavelength λ(read) excites fluorescence of F, probing this record. An optional third flash at a wavelength λ(reset) converts F back to D2, for a subsequent write-read cycle. The flash memory method offers the promise to decouple the recording of neural activity from its readout. In principle, the technique may enable one to generate snapshots of neural activity in a large volume of neural tissue, e.g., a complete mouse brain, by circumventing the challenge of imaging a large volume with simultaneous high spatial and high temporal resolution. The proof-of-principle flash memory sensors presented here will need improvements in sensitivity, speed, brightness, and membrane trafficking before this goal can be realized.

publication date

  • February 12, 2014

Date in CU Experts

  • January 30, 2018 5:20 AM

Full Author List

  • Venkatachalam V; Brinks D; Maclaurin D; Hochbaum D; Kralj J; Cohen AE

author count

  • 6

Other Profiles

Electronic International Standard Serial Number (EISSN)

  • 1520-5126

Additional Document Info

start page

  • 2529

end page

  • 2537


  • 136


  • 6