Bioassay-directed fractionation of mutagenic PAH atmospheric photooxidation products and ambient particulate extracts. Journal Article uri icon

Overview

abstract

  • Simulated atmospheric gas-phase reactions of naphthalene, fluorene and phenanthrene have been carried out in an environmental chamber with bioassay-directed chemical analysis of the reaction products. Nitro-PAH were found to be the most significant mutagens formed from the reactions of naphthalene and fluorene. The mutagram (bar graph of mutagenic activity versus HPLC fraction) of the phenanthrene reaction products closely resembled that of an ambient air particulate extract with the most mutagenic activity being in a fraction more polar than that in which the nitro-PAH elute. Nitrophenanthrene lactones (nitro-6H-dibenzo[b,d]pyran-6-ones) were found to account for the observed activity of this polar fraction of the phenanthrene reaction products. It has been shown that the utilization of an environmental chamber with a known PAH-starting material and the ability to produce sufficient product for isomer-specific identifications of mutagens is a promising complement to bioassay-directed fractionation of ambient air particulate extracts.

publication date

  • January 1, 1992

Full Author List

  • Arey J; Harger WP; Helmig D; Atkinson R

Other Profiles

Additional Document Info

start page

  • 67

end page

  • 76

volume

  • 281

issue

  • 1