A New Approach for Measuring Single-Cell Oxygen Consumption Rates. Journal Article uri icon

Overview

abstract

  • A novel system that has enabled the measurement of single-cell oxygen consumption rates is presented. The experimental apparatus includes a temperature controlled environmental chamber, an array of microwells etched in glass, and a lid actuator used to seal cells in the microwells. Each microwell contains an oxygen sensitive platinum phosphor sensor used to monitor the cellular metabolic rates. Custom automation software controls the digital image data collection for oxygen sensor measurements, which are analyzed using an image-processing program to yield the oxygen concentration within each microwell versus time. Two proof-of-concept experiments produced oxygen consumption rate measurements for A549 human epithelial lung cancer cells of 5.39 and 5.27 fmol/min/cell, closely matching published oxygen consumption rates for bulk A549 populations.

publication date

  • January 1, 2008

Full Author List

  • Molter TW; McQuaide SC; Holl MR; Meldrum DR; Dragavon JM; Anderson JB; Young AC; Burgess LW; Lidstrom ME

Other Profiles

Additional Document Info

start page

  • 32

end page

  • 42

volume

  • 5

issue

  • 1