Induction of Escherichia coli and Salmonella typhimurium into the viable but nonculturable state following chlorination of wastewater Journal Article uri icon



  • We examined the effects of chlorine disinfection on Escherichia coli and Salmonella typhimurium in secondary-treated wastewater to determine whether such treatment might induce these bacteria into the viable but nonculturable (VBNC) state. In this state, cells lose culturability but retain viability and the potential to revert to the metabolically active and infectious state. To examine the effects of chlorination on cells in different physiological states, cells from the logarithmic and stationary phases, or nutrient starved, or grown in natural wastewater, were studied. Isogenic cells with and without plasmids were also examined. Whereas a mixture of free and combined chlorine, as occurs under typical wastewater disinfection, was found to be rapidly lethal to most cells, regardless of their physiological state or plasmid content, c. 104 of the original 106 cells ml−1 did survive in the VBNC state. While we were not successful in resuscitating these cells to the culturable state, the presence of such nonculturable cells in treated wastewater offers a potential public health hazard.

publication date

  • September 1, 2005

has restriction

  • bronze

Date in CU Experts

  • November 5, 2020 9:12 AM

Full Author List

  • Oliver JD; Dagher M; Linden K

author count

  • 3

Other Profiles

International Standard Serial Number (ISSN)

  • 1477-8920

Electronic International Standard Serial Number (EISSN)

  • 1996-7829

Additional Document Info

start page

  • 249

end page

  • 257


  • 3


  • 3