Sensitive and Multiplexed On‐chip microRNA Profiling in Oil‐Isolated Hydrogel Chambers Journal Article uri icon



  • AbstractAlthough microRNAs (miRNAs) have been shown to be excellent indicators of disease state, current profiling platforms are insufficient for clinical translation. Here, we demonstrate a versatile hydrogel‐based microfluidic approach and novel amplification scheme for entirely on‐chip, sensitive, and highly specific miRNA detection without the risk of sequence bias. A simulation‐driven approach is used to engineer the hydrogel geometry and the gel‐reaction environment is chemically optimized for robust detection performance. The assay provides 22.6 fM sensitivity over a three log range, demonstrates multiplexing across at least four targets, and requires just 10.3 ng of total RNA input in a 2 hour and 15 minutes assay.

publication date

  • February 16, 2015

has restriction

  • closed

Date in CU Experts

  • January 27, 2021 5:03 AM

Full Author List

  • Lee H; Srinivas RL; Gupta A; Doyle PS

author count

  • 4

Other Profiles

International Standard Serial Number (ISSN)

  • 0044-8249

Electronic International Standard Serial Number (EISSN)

  • 1521-3757

Additional Document Info

start page

  • 2507

end page

  • 2511


  • 127


  • 8