Nonclassical C-glycosides, distinguished by their unique glycosidic bond connection mode, represent a promising avenue for the development of carbohydrate-based drugs. However, the accessibility of nonclassical C-glycosides hinders broader investigations into their structural features and modes of action. Herein, we present the first example of Pd-catalyzed stereospecific glycosylation of nonclassical anomeric stannanes with aryl or vinyl halides. This method furnishes desired nonclassical aryl and vinyl C-glycosides in good to excellent yields, while allowing for exclusive control of nonclassical anomeric configuration. Of significant note is the demonstration of the generality and practicality of this nonclassical C-glycosylation approach across more than 50 examples, encompassing various protected and unprotected saccharides, deoxy sugars, oligopeptides, and complex molecules. Furthermore, biological evaluation indicates that nonclassical C-glycosylation modifications of drug molecules can positively impact their biological activity. Additionally, extensive computational studies are conducted to elucidate the rationale behind differences in reaction reactivity, unveiling a transmetalation transition state containing silver (Ag) within a six-membered ring. Given its remarkable controllability, predictability, and consistently high chemical selectivity and stereospecificity regarding nonclassical anomeric carbon and Z/E configuration, the method outlined in this study offers a unique solution to the longstanding challenge of accessing nonclassical C-glycosides with exclusive stereocontrol.
