Imaging cellular responses to mechanical stimuli within three‐dimensional tissue constructs Journal Article uri icon



  • AbstractThe cellular response to environmental cues is complex, involving both structural and functional changes within the cell. Our understanding of this response is facilitated by microscopy techniques, but has been limited by our ability to image cell structure and function deep in highly‐scattering tissues or 3D constructs. A novel multimodal microscopy technique that combines coherent and incoherent imaging for simultaneous visualization of structural and functional properties of cells and engineered tissues is demonstrated. This microscopic technique allows for the simultaneous acquisition of optical coherence microscopy and multiphoton microscopy data with particular emphasis for applications in cell biology and tissue engineering. The capability of this technique is shown using representative 3D cell and tissue engineering cultures consisting of primary fibroblasts from transgenic green fluorescent protein (GFP) mice and GFP‐vinculin transfected fibroblasts. Imaging is performed following static and dynamic mechanically‐stimulating culture conditions. The microscopy technique presented here reveals unique complementary data on the structure and function of cells and their adhesions and interactions with the surrounding microenvironment. Microsc. Res. Tech., 2007. © 2007 Wiley‐Liss, Inc.

publication date

  • April 1, 2007

has restriction

  • closed

Date in CU Experts

  • January 15, 2014 11:28 AM

Full Author List

  • Tan W; Vinegoni C; Norman JJ; Desai TA; Boppart SA

author count

  • 5

Other Profiles

International Standard Serial Number (ISSN)

  • 1059-910X

Electronic International Standard Serial Number (EISSN)

  • 1097-0029

Additional Document Info

start page

  • 361

end page

  • 371


  • 70


  • 4