A method for evaluating microscope objectives to optimize performance of confocal systems Journal Article uri icon

Overview

abstract

  • SUMMARYA method for evaluating the performance of microscope objectives on two types of confocal scanning optical microscope is presented. Of these two confocal microscope types, off‐axis beam‐scanning systems are found to require microscope objectives which have been corrected for flatness of field as well as for spherical aberration and astigmatism in order to obtain maximum axial and laterial resolution. In the case of on‐axis specimen‐scanning microscopes, less highly corrected objective lenses (not corrected for flatness of field) may in practice prove to have superior resolving properties.

publication date

  • May 1, 1990

has restriction

  • closed

Date in CU Experts

  • April 4, 2014 12:08 PM

Full Author List

  • Cogswell CJ; Sheppard CJR; Moss MC; Howard CV

author count

  • 4

Other Profiles

International Standard Serial Number (ISSN)

  • 0022-2720

Electronic International Standard Serial Number (EISSN)

  • 1365-2818

Additional Document Info

start page

  • 177

end page

  • 185

volume

  • 158

issue

  • 2