Bacteriophage Qβ Replicase Contains the Protein Biosynthesis Elongation Factors EF Tu and EF Ts Journal Article uri icon

Overview

abstract

  • ; The enzyme, Qβ replicase, responsible for the replication of the RNA of; Escherichia coli; pahge Qβ, is composed of four nonidentical subunits, three of which, I, III, and IV, are coded for by the bacterial genome, while subunit II is phage-specific.; ; Subunit IV is shown to be identical to the protein synthesis elongation factor EF Ts by the following criteria: coelectrophoresis on polyacrylamide gels in sodium dodecyl sulfate and in urea buffers, identity of the first seven amino acids at the amino-terminus, precipitation of sub-unit IV by anti-EF T-factor serum, and stimulation of EF Tu-GDP exchange by subunit IV. Subunit III is shown to be identical to the protein synthesis elongation factor EF Tu by the following criteria: coelectrophoresis on sodium dodecyl sulfate gels, precipitation of EF Tu by anti-Qβ replicase serum, binding of guanine nucleotides, and binding of phenylalanyl-tRNA. In addition, Qβ replicase activity can be reconstituted from subunits I and II with EF Tu and EF Ts.

publication date

  • May 1, 1972

Date in CU Experts

  • April 22, 2014 10:56 AM

Full Author List

  • Blumenthal T; Landers TA; Weber K

author count

  • 3

Other Profiles

International Standard Serial Number (ISSN)

  • 0027-8424

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

Additional Document Info

start page

  • 1313

end page

  • 1317

volume

  • 69

issue

  • 5