An exon that prevents transport of a mature mRNA Journal Article uri icon



  • ; In; Caenorhabditis elegans; , pre-mRNA for the essential splicing factor U2AF; 65; sometimes is spliced to produce an RNA that includes an extra 216-bp internal exon, exon 3. Inclusion of exon 3 inserts an in-frame stop codon, yet this RNA is not subject to SMG-mediated RNA surveillance. To test whether exon 3 causes RNA to remain nuclear and thereby escape decay, we inserted it into the 3′ untranslated region of a; gfp; reporter gene. Although exon 3 did not affect accumulation or processing of the mRNA, it dramatically suppressed expression of green fluorescent protein (GFP). We showed by; in situ; hybridization that exon 3-containing; gfp; RNA is retained in the nucleus. Intriguingly, exon 3 contains 10 matches to the 8-bp 3′ splice-site consensus. We hypothesized that U2AF might recognize this octamer and thereby prevent export. This idea is supported by RNA interference experiments in which reduced levels of U2AF resulted in a small burst of; gfp; expression.;

publication date

  • March 30, 1999

has restriction

  • green

Date in CU Experts

  • April 24, 2014 9:17 AM

Full Author List

  • MacMorris MA; Zorio DAR; Blumenthal T

author count

  • 3

Other Profiles

International Standard Serial Number (ISSN)

  • 0027-8424

Electronic International Standard Serial Number (EISSN)

  • 1091-6490

Additional Document Info

start page

  • 3813

end page

  • 3818


  • 96


  • 7