Intercistronic Region Required for Polycistronic Pre-mRNA Processing in; Caenorhabditis elegans Journal Article uri icon



  • ABSTRACT; ; In; Caenorhabditis elegans; , polycistronic pre-mRNAs are processed by cleavage and polyadenylation at the 3′ ends of the upstream genes and; trans; splicing, generally to the specialized spliced leader SL2, at the 5′ ends of the downstream genes. Previous studies have indicated a relationship between these two events in the processing of a heat shock-induced; gpd-2–gpd-3; polycistronic pre-mRNA. Here, we report mutational analysis of the intercistronic region of this operon by linker scan analysis. Surprisingly, no sequences downstream of the 3′ end were important for 3′-end formation. In contrast, a U-rich (Ur) element located 29 bp downstream of the site of 3′-end formation was shown to be important for downstream mRNA biosynthesis. This ∼20-bp element is sufficient for SL2; trans; splicing and mRNA accumulation when transplanted to a heterologous context. Furthermore, when the downstream gene was replaced by a gene from another organism, no loss of; trans; -splicing specificity was observed, suggesting that the Ur element may be the primary signal required for downstream mRNA processing.;

publication date

  • February 15, 2001

Date in CU Experts

  • April 24, 2014 9:29 AM

Full Author List

  • Huang T; Kuersten S; Deshpande AM; Spieth J; MacMorris M; Blumenthal T

author count

  • 6

Other Profiles

International Standard Serial Number (ISSN)

  • 0270-7306

Electronic International Standard Serial Number (EISSN)

  • 1098-5549

Additional Document Info

start page

  • 1111

end page

  • 1120


  • 21


  • 4