BACTERIOPHAGE T4 DNA-DEPENDENT; in vitro; SYNTHESIS OF LYSOZYME Journal Article

Overview

; A cell-free system derived from uninfected; Escherichia coli; previously was shown to synthesize β-glucosyl transferase in response to T4 DNA. This same; in vitro; system, when incubated at slightly higher magnesium concentrations, also synthesized enzymatically active lysozyme. The lysozyme activity that appeared was judged to be T4-specific since antibodies prepared against authentic T4-lysozyme inactivated the; in vitro; synthesized enzyme. DNA from a T4 mutant carrying a deletion in the lysozyme gene stimulated amino acid incorporation to the same extent as wild-type T4 DNA but was inactive in directing the synthesis of lysozyme. Various inhibitors of RNA and protein synthesis inhibited the; in vitro; synthesis of lysozyme.;