The yeast Apq12 protein affects nucleocytoplasmic mRNA transport Journal Article uri icon

Overview

abstract

  • An important step in mRNA biogenesis is the export of mRNA from the nucleus to the cytoplasm. In this work, we provide evidence that the previously uncharacterized gene APQ12 functions in nucleocytoplasmic mRNA transport in Saccharomyces cerevisiae. First, apq12Δ strains manifest 3′ hyperadenylated mRNA similar to other previously characterized RNA export mutants. Second, bulk poly(A)+ RNA is retained in the nucleus in apq12Δ cells. Third, an Apq12p–GFP chimeric protein is localized to the nuclear periphery. Fourth, mRNA in apq12Δ cells is stabilized, consistent with a defect in the rate of nuclear export. Interestingly, apq12Δ mutants are severely compromised for growth and display atypical cell morphology. Because this aberrant cell morphology is not seen with other viable export mutants, Apq12p must have either an additional cellular function, or preferentially impinge on the export of mRNAs regulating cell growth. Together, these findings support a role for APQ12 in nucleocytoplasmic transport of mRNA.

publication date

  • September 1, 2004

Date in CU Experts

  • January 27, 2015 11:54 AM

Full Author List

  • BAKER KE; COLLER J; PARKER ROY

author count

  • 3

published in

  • RNA  Journal

Other Profiles

International Standard Serial Number (ISSN)

  • 1355-8382

Electronic International Standard Serial Number (EISSN)

  • 1469-9001

Additional Document Info

start page

  • 1352

end page

  • 1358

volume

  • 10

issue

  • 9