The RNase Rny1p cleaves tRNAs and promotes cell death during oxidative stress in Saccharomyces cerevisiae Journal Article uri icon



  • The cellular response to stress conditions involves a decision between survival or cell death when damage is severe. A conserved stress response in eukaryotes involves endonucleolytic cleavage of transfer RNAs (tRNAs). The mechanism and significance of such tRNA cleavage is unknown. We show that in yeast, tRNAs are cleaved by the RNase T2 family member Rny1p, which is released from the vacuole into the cytosol during oxidative stress. Rny1p modulates yeast cell survival during oxidative stress independently of its catalytic ability. This suggests that upon release to the cytosol, Rny1p promotes cell death by direct interactions with downstream components. Thus, detection of Rny1p, and possibly its orthologues, in the cytosol may be a conserved mechanism for assessing cellular damage and determining cell survival, analogous to the role of cytochrome c as a marker for mitochondrial damage.

publication date

  • April 6, 2009

has restriction

  • hybrid

Date in CU Experts

  • January 27, 2015 11:56 AM

Full Author List

  • Thompson DM; Parker R

author count

  • 2

Other Profiles

International Standard Serial Number (ISSN)

  • 0021-9525

Electronic International Standard Serial Number (EISSN)

  • 1540-8140

Additional Document Info

start page

  • 43

end page

  • 50


  • 185


  • 1