[3'-32P]-labeling tRNA with nucleotidyltransferase for assaying aminoacylation and peptide bond formation. Journal Article uri icon

Overview

abstract

  • The analysis of reactions involving amino acids esterified to tRNAs traditionally uses radiolabeled amino acids. We describe here an alternative assay involving [3'-32P]-labeled tRNA followed by nuclease digestion and TLC analysis that permits aminoacylation to be monitored in an efficient, quantitative manner while circumventing many of the problems faced when using radiolabeled amino acids. We also describe a similar assay using [3'-32P]-labeled aa-tRNAs to determine the rate of peptide bond formation on the ribosome. This type of assay can also potentially be adapted to study other reactions involving an amino acid or peptide esterified to tRNA.

publication date

  • February 1, 2008

Date in CU Experts

  • March 13, 2015 12:56 PM

Full Author List

  • Ledoux S; Uhlenbeck OC

author count

  • 2

citation count

  • 27

Other Profiles

International Standard Serial Number (ISSN)

  • 1046-2023

Electronic International Standard Serial Number (EISSN)

  • 1095-9130

Additional Document Info

start page

  • 74

end page

  • 80

volume

  • 44

issue

  • 2