An introduction to best practices in free energy calculations. Journal Article uri icon



  • Free energy calculations are extremely useful for investigating small-molecule biophysical properties such as protein-ligand binding affinities and partition coefficients. However, these calculations are also notoriously difficult to implement correctly. In this chapter, we review standard methods for computing free energy via simulation, discussing current best practices and examining potential pitfalls for computational researchers performing them for the first time. We include a variety of examples and tips for how to set up and conduct these calculations, including applications to relative binding affinities and small-molecule solvation free energies.

publication date

  • January 1, 2013

has restriction

  • closed

Date in CU Experts

  • September 4, 2015 2:15 AM

Full Author List

  • Shirts MR; Mobley DL

author count

  • 2

Other Profiles

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Additional Document Info

start page

  • 271

end page

  • 311


  • 924