Spectral image scanning microscopy. Journal Article uri icon

Overview

abstract

  • For decades, the confocal microscope has represented one of the dominant imaging systems in biomedical imaging at sub-cellular lengthscales. Recently, however, it has increasingly been replaced by a related, but more powerful successor technique termed image scanning microscopy (ISM). In this article, we present ISM capable of measuring spectroscopic information such as that contained in fluorescence or Raman images. Compared to established confocal spectroscopic imaging systems, our implementation offers similar spectral resolution, but higher spatial resolution and detection efficiency. Color sensitivity is achieved by a grating placed in the detection path in conjunction with a camera collecting both spatial and spectral information. The multidimensional data is processed using multi-view maximum likelihood image reconstruction. Our findings are supported by numerical simulations and experiments on micro beads and double-stained HeLa cells.

publication date

  • May 1, 2019

Full Author List

  • Strasser F; Offterdinger M; Piestun R; Jesacher A

Other Profiles

Additional Document Info

start page

  • 2513

end page

  • 2527

volume

  • 10

issue

  • 5