Spectral image scanning microscopy. Journal Article uri icon



  • For decades, the confocal microscope has represented one of the dominant imaging systems in biomedical imaging at sub-cellular lengthscales. Recently, however, it has increasingly been replaced by a related, but more powerful successor technique termed image scanning microscopy (ISM). In this article, we present ISM capable of measuring spectroscopic information such as that contained in fluorescence or Raman images. Compared to established confocal spectroscopic imaging systems, our implementation offers similar spectral resolution, but higher spatial resolution and detection efficiency. Color sensitivity is achieved by a grating placed in the detection path in conjunction with a camera collecting both spatial and spectral information. The multidimensional data is processed using multi-view maximum likelihood image reconstruction. Our findings are supported by numerical simulations and experiments on micro beads and double-stained HeLa cells.

publication date

  • May 1, 2019

has restriction

  • gold

Date in CU Experts

  • June 2, 2019 6:19 AM

Full Author List

  • Strasser F; Offterdinger M; Piestun R; Jesacher A

author count

  • 4

Other Profiles

International Standard Serial Number (ISSN)

  • 2156-7085

Additional Document Info

start page

  • 2513

end page

  • 2527


  • 10


  • 5