Evidence for facilitated lactate uptake in lizard skeletal muscle. Journal Article uri icon

Overview

abstract

  • To understand more fully lactate metabolism in reptilian muscle, lactate uptake in lizard skeletal muscle was measured and its similarities to the monocarboxylate transport system found in mammals were examined. At 2 min, uptake rates of 15 mmol l(-1) lactate into red iliofibularis (rIF) were 2.4- and 2.2-fold greater than white iliofibularis (wIF) and mouse soleus, respectively. alpha-Cyano-4-hydroxycinnamate (15 mmol l(-1)) caused little inhibition of uptake in wIF but caused a 42-54 % reduction in the uptake rate of lactate into rIF, suggesting that much of the lactate uptake by rIF is via protein-mediated transport. N-ethymaleimide (ETH) (10 mmol l(-1)) also caused a reduction in the rate of uptake, but measurements of adenylate and phosphocreatine concentrations show that ETH had serious effects on rIF and wIF and may not be appropriate for transport inhibition studies in reptiles. The higher net uptake rate by rIF than by wIF agrees with the fact that rIF shows much higher rates of lactate utilization and incorporation into glycogen than wIF. This study also suggests that lactate uptake by reptilian muscle is similar to that by mammalian muscle and that, evolutionarily, this transport system may be relatively conserved even in animals with very different patterns of lactate metabolism.

publication date

  • December 1, 2001

Date in CU Experts

  • September 9, 2013 9:28 AM

Full Author List

  • Donovan ER; Gleeson TT

author count

  • 2

citation count

  • 4

Other Profiles

International Standard Serial Number (ISSN)

  • 0022-0949

Electronic International Standard Serial Number (EISSN)

  • 1477-9145

Additional Document Info

start page

  • 4099

end page

  • 4106

volume

  • 204

issue

  • Pt 23