Fast finite difference solver for optical microscopy in deep biological tissue | CU Experts

Optical scattering poses a significant challenge to high-resolution; microscopy within deep tissue. To accurately predict the performance; of various microscopy techniques in thick samples, we present a; computational model that efficiently solves Maxwell’s equation in; highly scattering media. This toolkit simulates the deterioration of; the laser beam point spread function (PSF) without making a paraxial; approximation, enabling accurate modeling of high-numerical-aperture; (NA) objective lenses commonly employed in experiments. Moreover, this; framework is applicable to a broad range of scanning microscopy; techniques including confocal microscopy, stimulated emission; depletion (STED) microscopy, and ground-state depletion microscopy.; Notably, the proposed method requires only readily obtainable; macroscopic tissue parameters. As a practical demonstration, we; investigate the performance of Laguerre–Gaussian (LG) versus; Hermite–Gaussian (HG) depletion beams in STED microscopy.

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