Chaperone-mediated in vitro disassembly of polyoma- and papillomaviruses. Journal Article uri icon

Overview

abstract

  • Hsp70 chaperones play a role in polyoma- and papillomavirus assembly, as evidenced by their interaction in vivo with polyomavirus capsid proteins at latetimes after virus infection and by their ability to assemble viral capsomeres into capsids in vitro. We studied whether Hsp70 chaperones might also participate in the uncoating reaction. In vivo, Hsp70 co-immunoprecipitated with polyomavirus virion VP1 at 3 h after infection of mouse cells. In vitro, prokaryotic and eukaryotic Hsp70 chaperones efficiently disassembled polyoma- and papillomavirus-like particles and virions in energy-dependent reactions. These observations support a role for cell chaperones in the disassembly of these viruses.

publication date

  • January 1, 2006

Full Author List

  • Chromy LR; Oltman A; Estes PA; Garcea RL

Additional Document Info

start page

  • 5086-91

end page

  • 5086-91

volume

  • 80