Recent advances in stimulated emission depletion (STED) super-resolution microscopy for; in vivo; imaging
Journal Article
Overview
abstract
Recent advances in stimulated emission depletion (STED) microscopy have resulted in breakthroughs in biological imaging. Such advances include three-dimensional (3D) imaging, aberration correction to enhance resolution at depth in tissue, reduction in photobleaching, and label-free STED-inspired alternatives, enabling in vivo applications. In this perspective, we first summarize the basic principles of stimulated emission depletion microscopy and its major challenges. Next, we discuss techniques to reduce photobleaching, 3D imaging with STED, deep tissue imaging with STED, fiber-coupled STED, live animal imaging, and label-free imaging with super-resolution. Finally, we summarize and provide perspectives for future research.
