The mechanism of interaction between focused ultrasound and microbubbles in blood-brain barrier opening in mice Journal Article uri icon



  • The activation of bubbles by an acoustic field has been shown to temporarily open the blood-brain barrier (BBB), but the trigger cause responsible for the physiological effects involved in the process of BBB opening remains unknown. Here, the trigger cause (i.e., physical mechanism) of the focused ultrasound-induced BBB opening with monodispersed microbubbles is identified. Sixty-seven mice were injected intravenously with bubbles of 1–2, 4–5, or 6–8 μm in diameter and the concentration of 107 numbers/ml. The right hippocampus of each mouse was then sonicated using focused ultrasound (1.5 MHz frequency, 100 cycles pulse length, 10 Hz pulse repetition frequency, 1 min duration). Peak-rarefactional pressures of 0.15, 0.30, 0.45, or 0.60 MPa were applied to identify the threshold of BBB opening and inertial cavitation (IC). Our results suggest that the BBB opens with nonlinear bubble oscillation when the bubble diameter is similar to the capillary diameter and with inertial cavitation when it is not. The bubble may thus have to be in contact with the capillary wall to induce BBB opening without IC. BBB opening was shown capable of being induced safely with nonlinear bubble oscillation at the pressure threshold and its volume was highly dependent on both the acoustic pressure and bubble diameter.

publication date

  • November 1, 2011

has restriction

  • green

Date in CU Experts

  • December 10, 2013 9:08 AM

Full Author List

  • Tung Y-S; Vlachos F; Feshitan JA; Borden MA; Konofagou EE

author count

  • 5

Other Profiles

International Standard Serial Number (ISSN)

  • 0001-4966

Electronic International Standard Serial Number (EISSN)

  • 1520-8524

Additional Document Info

start page

  • 3059

end page

  • 3067


  • 130


  • 5