Multiple independent domains of dGW182 function in miRNA-mediated repression in Drosophila Journal Article uri icon



  • miRNA-mediated repression affects a wide range of biological processes including development and human pathologies. The GW182 protein is a key component of miRNA repression complex, recruited by Argonaute and functioning downstream to repress translation and accelerate mRNA degradation, but little is known about how GW182 proteins act. Using both tethered function and complementation assays, we identify three independent domains of the Drosophila GW182 protein (also termed Gawky) that are sufficient to repress mRNA. Each of these domains also functions independently of poly(A) tails. These results indicate that miRNA-mediated repression is facilitated by multiple domains of GW182.

publication date

  • May 1, 2009

has restriction

  • bronze

Date in CU Experts

  • February 20, 2014 11:14 AM

Full Author List

  • Chekulaeva M; Filipowicz W; Parker R

author count

  • 3

published in

  • RNA  Journal

Other Profiles

International Standard Serial Number (ISSN)

  • 1355-8382

Electronic International Standard Serial Number (EISSN)

  • 1469-9001

Additional Document Info

start page

  • 794

end page

  • 803


  • 15


  • 5