tRNA cleavage is a conserved response to oxidative stress in eukaryotes Journal Article uri icon



  • Recent results have identified a diversity of small RNAs in a wide range of organisms. In this work, we demonstrate that Saccharomyces cerevisiae contains a small RNA population consisting primarily of tRNA halves and rRNA fragments. Both 5′ and 3′ fragments of tRNAs are detectable by Northern blot analysis, suggesting a process of endonucleolytic cleavage. tRNA and rRNA fragment production in yeast is most pronounced during oxidative stress conditions, especially during entry into stationary phase. Similar tRNA fragments are also observed in human cell lines and in plants during oxidative stress. These results demonstrate that tRNA cleavage is a conserved aspect of the response to oxidative stress.

publication date

  • October 1, 2008

has restriction

  • bronze

Date in CU Experts

  • February 20, 2014 11:20 AM

Full Author List

  • Thompson DM; Lu C; Green PJ; Parker R

author count

  • 4

published in

  • RNA  Journal

Other Profiles

International Standard Serial Number (ISSN)

  • 1355-8382

Electronic International Standard Serial Number (EISSN)

  • 1469-9001

Additional Document Info

start page

  • 2095

end page

  • 2103


  • 14


  • 10