The DEAD-Box RNA Helicase Ded1p Affects and Accumulates inSaccharomyces cerevisiaeP-Bodies Journal Article uri icon

Overview

abstract

  • Recent results suggest that cytoplasmic mRNAs can form translationally repressed messenger ribonucleoprotein particles (mRNPs) capable of decapping and degradation, or accumulation into cytoplasmic processing bodies (P-bodies), which can function as sites of mRNA storage. The proteins that function in transitions between the translationally repressed mRNPs that accumulate in P-bodies and mRNPs engaged in translation are largely unknown. Herein, we demonstrate that the yeast translation initiation factor Ded1p can localize to P-bodies. Moreover, depletion of Ded1p leads to defects in P-body formation. Overexpression of Ded1p results in increased size and number of P-bodies and inhibition of growth in a manner partially suppressed by loss of Pat1p, Dhh1p, or Lsm1p. Mutations that inactivate the ATPase activity of Ded1p increase the overexpression growth inhibition of Ded1p and prevent Ded1p from localizing in P-bodies. Combined with earlier work showing Ded1p can have a positive effect on translation, these results suggest that Ded1p is a bifunctional protein that can affect both translation initiation and P-body formation.

publication date

  • March 1, 2008

Date in CU Experts

  • February 20, 2014 11:32 AM

Full Author List

  • Beckham C; Hilliker A; Cziko A-M; Noueiry A; Ramaswami M; Parker R

author count

  • 6

Other Profiles

International Standard Serial Number (ISSN)

  • 1059-1524

Electronic International Standard Serial Number (EISSN)

  • 1939-4586

Additional Document Info

start page

  • 984

end page

  • 993

volume

  • 19

issue

  • 3