Analysis of P-Body Assembly inSaccharomyces cerevisiae Journal Article uri icon

Overview

abstract

  • Recent experiments have defined cytoplasmic foci, referred to as processing bodies (P-bodies), that contain untranslating mRNAs in conjunction with proteins involved in translation repression and mRNA decapping and degradation. However, the order of protein assembly into P-bodies and the interactions that promote P-body assembly are unknown. To gain insight into how yeast P-bodies assemble, we examined the P-body accumulation of Dcp1p, Dcp2p, Edc3p, Dhh1p, Pat1p, Lsm1p, Xrn1p, Ccr4p, and Pop2p in deletion mutants lacking one or more P-body component. These experiments revealed that Dcp2p and Pat1p are required for recruitment of Dcp1p and of the Lsm1-7p complex to P-bodies, respectively. We also demonstrate that P-body assembly is redundant and no single known component of P-bodies is required for P-body assembly, although both Dcp2p and Pat1p contribute to P-body assembly. In addition, our results indicate that Pat1p can be a nuclear-cytoplasmic shuttling protein and acts early in P-body assembly. In contrast, the Lsm1-7p complex appears to primarily function in a rate limiting step after P-body assembly in triggering decapping. Taken together, these results provide insight both into the function of individual proteins involved in mRNA degradation and the mechanisms by which yeast P-bodies assemble.

publication date

  • June 1, 2007

has restriction

  • green

Date in CU Experts

  • February 20, 2014 11:38 AM

Full Author List

  • Teixeira D; Parker R

author count

  • 2

Other Profiles

International Standard Serial Number (ISSN)

  • 1059-1524

Electronic International Standard Serial Number (EISSN)

  • 1939-4586

Additional Document Info

start page

  • 2274

end page

  • 2287

volume

  • 18

issue

  • 6