Cytoplasmic Decay of Intergenic Transcripts in; Saccharomyces cerevisiae Journal Article uri icon



  • ABSTRACT; ; Eukaryotes produce a number of noncoding transcripts from intergenic regions. In; Saccharomyces cerevisiae; , such cryptic unstable transcripts (CUTs) are thought to be degraded in the nucleus by a process involving polyadenylation and 3′-to-5′ degradation by the nuclear exosome. In this work, we examine the degradation pathway of the RNA; SRG1; , which is produced from an intergenic region and contributes to the regulation of the; SER3; gene by promoter occlusion during; SRG1; transcription. Although there is some effect on; SRG1; transcript levels when the nuclear exosome is compromised, the bulk of the; SRG1; RNA is degraded in the cytoplasm by decapping and 5′-to-3′ exonucleolytic digestion. Examination of other CUTs suggests that individual CUTs can be degraded by a variety of different mechanisms, including nuclear decay, cytoplasmic decapping and 5′-to-3′ decay, and nonsense-mediated decay. Moreover, some CUTs appear to be associated with polyribosomes. These results indicate that some CUTs can be exported from the nucleus and enter translation before being degraded, identifying a potential mechanism for the evolution of new protein-encoding genes.;

publication date

  • January 1, 2007

Date in CU Experts

  • February 20, 2014 11:42 AM

Full Author List

  • Thompson DM; Parker R

author count

  • 2

Other Profiles

International Standard Serial Number (ISSN)

  • 0270-7306

Electronic International Standard Serial Number (EISSN)

  • 1098-5549

Additional Document Info

start page

  • 92

end page

  • 101


  • 27


  • 1