Crystal structure of Ski8p, a WD‐repeat protein with dual roles in mRNA metabolism and meiotic recombination Journal Article uri icon



  • AbstractSki8p is a WD‐repeat protein with an essential role for the Ski complex assembly in an exosome‐dependent 3′‐to‐5′ mRNA decay. In addition, Ski8p is involved in meiotic recombination by interacting with Spo11p protein. We have determined the crystal structure of Ski8p from Saccharomyces cerevisiae at 2.2 Å resolution. The structure reveals that Ski8p folds into a seven‐bladed β propeller. Mapping sequence conservation and hydrophobicities of amino acids on the molecular surface of Ski8p reveals a prominent site on the top surface of the β propeller, which is most likely involved in mediating interactions of Ski8p with Ski3p and Spo11p. Mutagenesis combined with yeast two‐hybrid and GST pull‐down assays identified the top surface of the β propeller as being required for Ski8p binding to Ski3p and Spo11p. The functional implications for Ski8p function in both mRNA decay and meiotic recombination are discussed.

publication date

  • October 1, 2004

has restriction

  • green

Date in CU Experts

  • February 21, 2014 12:05 PM

Full Author List

  • Cheng Z; Liu Y; Wang C; Parker R; Song H

author count

  • 5

Other Profiles

International Standard Serial Number (ISSN)

  • 0961-8368

Electronic International Standard Serial Number (EISSN)

  • 1469-896X

Additional Document Info

start page

  • 2673

end page

  • 2684


  • 13


  • 10