Sequence-Specific Binding of Transfer RNA by Glyceraldehyde-3-Phosphate Dehydrogenase Journal Article uri icon

Overview

abstract

  • A transfer RNA (tRNA) binding protein present in HeLa cell nuclear extracts was purified and identified as the glycolytic enzyme glyceraldehyde-3-phosphate dehydrogenase (GAPDH). Studies with mutant tRNAs indicated that GAPDH recognizes both sequence and structural features in the RNA. GAPDH discriminated between wild-type tRNA and two tRNA mutants that are defective in nuclear export, which suggests that the protein may participate in RNA export. The cofactor nicotinamide adenine dinucleotide disrupted complex formation between tRNA and GAPDH and thus may share a common binding site with the RNA. Indirect immunofluorescence experiments showed that GAPDH is present in the nucleus as well as in the cytoplasm.

publication date

  • January 15, 1993

has restriction

  • closed

Date in CU Experts

  • May 16, 2014 10:18 AM

Full Author List

  • Singh R; Green MR

author count

  • 2

Other Profiles

International Standard Serial Number (ISSN)

  • 0036-8075

Electronic International Standard Serial Number (EISSN)

  • 1095-9203

Additional Document Info

start page

  • 365

end page

  • 368

volume

  • 259

issue

  • 5093