Verifying the function and localization of genetically encoded Ca2+ sensors and converting FRET ratios to Ca2+ concentrations. Journal Article uri icon

Overview

abstract

  • Genetically encoded, ratiometric, fluorescent Ca(2+) biosensors can be used in living cells to quantitatively measure free Ca(2+) concentrations in the cytosol or in organelles. This protocol describes how to perform a calibration of a Ca(2+) sensor expressed in cultured mammalian cells as images are acquired using a widefield fluorescence microscope. This protocol also explains how to calculate Förster resonance energy transfer (FRET) ratios from acquired images and how to convert FRET ratios to Ca(2+) concentrations.

publication date

  • January 1, 2015

Full Author List

  • Park JG; Palmer AE

Other Profiles

Additional Document Info

start page

  • pdb.prot076547

volume

  • 2015

issue

  • 1