Cardiomyocyte preparation, culture, and gene transfer. Journal Article uri icon

Overview

abstract

  • Neonatal rat ventricular myocytes (NRVMs) cultured in vitro have been used as a model system for easily recreating and studying several cardiac molecular conditions, such as hypertrophy, oxygen deprivation, and gene expression. However, low efficiency of gene transfer has often represented one of the major limitations of this technique. In this chapter we describe in detail how to isolate NRVMs from neonatal rat heart and the optimal conditions for their long-term culture. Different cardiomyocyte transfection methodologies, based on viral or viral/chemical delivery carriers, are also discussed.

publication date

  • January 1, 2007

Date in CU Experts

  • March 13, 2015 1:10 AM

Full Author List

  • Maass AH; Buvoli M

author count

  • 2

citation count

  • 16

Other Profiles

International Standard Serial Number (ISSN)

  • 1064-3745

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Additional Document Info

start page

  • 321

end page

  • 330

volume

  • 366