Synthesis of mixed sequence borane phosphonate DNA. Journal Article uri icon

Overview

abstract

  • A novel solid-phase phosphoramidite-based method has been developed for the synthesis of borane phosphonate DNA. Keys to this new approach are replacement of the common 5'-dimethoxytrityl blocking group with a 5'-silyl ether and the use of new protecting groups on the bases (adenine, N6-dimethoxytrityl; cytosine, N4-trimethoxytrityl; guanine, N2-[9-fluorenylmethoxycarbonyl]; thymine, N3-anisoyl). Because of these developments, it is now possible for the first time to synthesize oligodeoxynucleotides having any combination of the four 2'-deoxynucleosides and both phosphate and borane phosphonate internucleotide linkages (including oligomers having exclusively borane phosphonate linkages).

publication date

  • June 1, 2006

Full Author List

  • McCuen HB; NoĆ© MS; Sierzchala AB; Higson AP; Caruthers MH

Other Profiles

Additional Document Info

start page

  • 8138

end page

  • 8139

volume

  • 128

issue

  • 25