In vivo assembly of the tau-complex of the DNA polymerase III holoenzyme expressed from a five-gene artificial operon. Cleavage of the tau-complex to form a mixed gamma-tau-complex by the OmpT protease. Journal Article uri icon

Overview

abstract

  • A plasmid was constructed that encodes all five subunits of the Escherichia coli tau-complex on a single artificially constructed operon under the control of an inducible promoter. The proteins tau, delta, delta , chi, and psi overproduced from this artificial operon assemble efficiently in vivo, providing an efficient source of homogeneous tau-complex. The gamma subunit is a truncated form of tau that is produced by a translational frameshift. When protein expression was induced in bacterial strains containing the outer membrane protein T (OmpT) protease, tau was proteolyzed after lysis to a gamma-like protein, gammaP, and a peptide, C-tau, corresponding to the C terminus of tau. N-terminal sequencing of C-tau revealed a cleavage site between two lysines at positions 429 and 430 of tau. The deduced sequence of gammaP is, therefore, only two amino acids shorter than natural gamma. The proteolysis by OmpT was also shown directly by using purified OmpT and tau-complex in an in vitro reaction. A gammaP-complex and a mixed tau-gammaP-complex were purified from ompT+ cells. When the tau-complex proteins were overexpressed in ompT- bacteria, intact tau-complex lacking gammaP could be purified.

publication date

  • April 1, 1996

has subject area

Date in CU Experts

  • October 1, 2013 12:09 PM

Full Author List

  • Pritchard AE; Dallmann HG; McHenry CS

author count

  • 3

citation count

  • 13

Other Profiles

International Standard Serial Number (ISSN)

  • 0021-9258

Electronic International Standard Serial Number (EISSN)

  • 1083-351X

Additional Document Info

start page

  • 10291

end page

  • 10298

volume

  • 271

issue

  • 17