Tau protects beta in the leading-strand polymerase complex at the replication fork. Journal Article uri icon

Overview

abstract

  • Replication forks formed in the absence of the tau subunit of the DNA polymerase III holoenzyme produce shorter leading and lagging strands than when tau is present. We show that one reason for this is that in the absence of tau, but in the presence of the gamma-complex, leading-strand synthesis is no longer highly processive. In the absence of tau, the size of the leading strand becomes proportional to the concentration of beta and inversely proportional to the concentration of the gamma-complex. In addition, the beta in the leading-strand complex is no longer resistant to challenge by either anti-beta antibodies or poly(dA):oligo(dT). Thus, tau is required to cement a processive leading-strand complex, presumably by preventing removal of beta catalyzed by the gamma-complex.

publication date

  • February 1, 1996

Date in CU Experts

  • October 1, 2013 12:09 PM

Full Author List

  • Kim S; Dallmann HG; McHenry CS; Marians KJ

author count

  • 4

citation count

  • 18

Other Profiles

International Standard Serial Number (ISSN)

  • 0021-9258

Electronic International Standard Serial Number (EISSN)

  • 1083-351X

Additional Document Info

start page

  • 4315

end page

  • 4318

volume

  • 271

issue

  • 8