Coupling of a replicative polymerase and helicase: a tau-DnaB interaction mediates rapid replication fork movement. Journal Article uri icon

Overview

abstract

  • The E. coli replication fork synthesizes DNA at the rate of nearly 1000 nt/s. We show here that an interaction between the tau subunit of the replicative polymerase (the DNA polymerase III holoenzyme) and the replication fork DNA helicase (DnaB) is required to mediate this high rate of replication fork movement. In the absence of this interaction, the polymerase follows behind the helicase at a rate equal to the slow (approximately 35 nt/s) unwinding rate of the helicase alone, whereas upon establishing a tau-DnaB contact, DnaB becomes a more effective helicase, increasing its translocation rate by more than 10-fold. This finding establishes the existence of both a physical and communications link between the two major replication machines in the replisome: the DNA polymerase and the primosome.

publication date

  • February 1, 1996

Date in CU Experts

  • October 1, 2013 12:09 PM

Full Author List

  • Kim S; Dallmann HG; McHenry CS; Marians KJ

author count

  • 4

citation count

  • 144

published in

Other Profiles

International Standard Serial Number (ISSN)

  • 0092-8674

Electronic International Standard Serial Number (EISSN)

  • 1097-4172

Additional Document Info

start page

  • 643

end page

  • 650

volume

  • 84

issue

  • 4