Identification, isolation, and overexpression of the gene encoding the psi subunit of DNA polymerase III holoenzyme. Journal Article uri icon



  • The gene encoding the psi subunit of DNA polymerase III holoenzyme, holD, was identified and isolated by an approach in which peptide sequence data were used to obtain a DNA hybridization probe. The gene, which maps to 99.3 centisomes, was sequenced and found to be identical to a previously uncharacterized open reading frame that overlaps the 5' end of rimI by 29 bases, contains 411 bp, and is predicted to encode a protein of 15,174 Da. When expressed in a plasmid that also expressed holC, holD directed expression of the psi subunit to about 3% of total soluble protein.

publication date

  • September 1, 1993

has subject area

has restriction

  • green

Date in CU Experts

  • October 1, 2013 12:09 PM

Full Author List

  • Carter JR; Franden MA; Aebersold R; McHenry CS

author count

  • 4

Other Profiles

International Standard Serial Number (ISSN)

  • 0021-9193

Additional Document Info

start page

  • 5604

end page

  • 5610


  • 175


  • 17