Determination of the precise location and orientation of the Escherichia coli dnaE gene. Journal Article uri icon

Overview

abstract

  • The minimal region required for expression of the dnaE gene of Escherichia coli has been determined relative to a detailed restriction endonuclease map. This has been accomplished by analysis of Bal 31 exonuclease-generated deletions from the termini of the E. coli DNA contained in plasmid pMWE303 , a plasmid that we have previously demonstrated to contain the dnaE gene (M. M. Welch and C. S. McHenry , J. Bacteriol . 152:351-356, 1982). The competence of these deletion-containing plasmids in expressing the alpha subunit of DNA polymerase III holoenzyme has been determined by their ability both to complement a dnaE mutant and to direct the synthesis of a complete alpha subunit. The carboxyl-terminal coding region of dnaE has been identified through the detection of partial alpha polypeptides encoded by plasmids containing deletions from one end of the gene. This approach has permitted the precise determination of both termini of the dnaE gene and the determination of the orientation of the gene within the E. coli chromosome.

publication date

  • May 1, 1984

Date in CU Experts

  • October 1, 2013 12:09 PM

Full Author List

  • Shepard D; Oberfelder RW; Welch MM; McHenry CS

author count

  • 4

citation count

  • 12

Other Profiles

International Standard Serial Number (ISSN)

  • 0021-9193

Electronic International Standard Serial Number (EISSN)

  • 1098-5530

Additional Document Info

start page

  • 455

end page

  • 459

volume

  • 158

issue

  • 2