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Publications in VIVO

Sousa, Marcelo C

Professor

Positions

Research Areas research areas

Research

research overview

  • Dr. Sousa uses a multidisciplinary research approach, with X-ray crystallography to obtain high-resolution structures and complementary biochemical and biophysical studies to understand, in molecular detail, cellular processes including: (i) Bacterial and Mitochondrial Outer Membrane Biogenesis: focused on how chaperones and the BAM membrane protein complex cooperate to promote folding and specific insertion of beta-barrel OMPs into bacterial and mitochondrial outer membranes. (ii) Modification of the Bacterial Outer Membranes Mediating Antibiotic Resistance: Lipid-A modification with Ara4N confers resistance to antibiotics such as colistin. Dr. Sousa’s group determines the structure and mechanism of enzymes essential for this modification as a key for the design of specific inhibitors of antibiotic resistance. (iii) Protein Secretion Systems: Focused on understanding for bacterial protein secretion and its repurposing for delivery of engineered proteins to the host.

keywords

  • Structural Biology

Publications

selected publications

Teaching

courses taught

  • BCHM 1020 - A Path to Success: Introduction to the Biochemistry Major
    Primary Instructor - Fall 2024
    This course will help students navigate their first year of college and develop the skills needed for academic success as Biochemistry majors. Students will improve academic strategies (i.e. time management, problem-solving and study skills), identify available campus resources, learn how to get involved in clubs and gain research experience, and explore career opportunities as a biochemistry graduate. This is a first-year elective course specifically designed for first year and other students exploring their educational and career opportunities. Recommended restriction: New BCHM majors.
  • BCHM 4761 - Biochemistry Laboratory
    Primary Instructor - Spring 2022 / Spring 2023
    Two 4-hour periods per week. Introduction to modern biochemical techniques. Topics include enzymology, spectrophotometry, electrophoresis, multi-step protein purification, recombinant DNA techniques and molecular cloning. Formerly CHEM 4761.
  • BCHM 5770 - Fundamentals of Biochemistry I
    Primary Instructor - Fall 2019
    Introduction to conducting research in Biochemistry, including covering foundational topics in Biochemistry that include concepts such as protein structure and function, experimental approaches to study biochemical processes, and analysis and interpretation of data. Topics will be taught through the perspective of the scientific literature with an emphasis on critical analysis of research. Additional subject areas will include discussions of aspects of conducting biochemical research in academic and industrial settings. Intended for entering Biochemistry graduate students. Formerly CHEM 5770.
  • BCHM 5771 - Advanced General Biochemistry 1
    Primary Instructor - Fall 2019 / Fall 2020
    Lect. In-depth analysis of DNA structure and replication, RNA synthesis and processing, protein synthesis, enzyme function and mechanism, protein structure, protein dynamics, and physical chemistry of macromolecules. Intended as a comprehensive treatment of areas central to modern biochemistry for entering graduate students. Formerly CHEM 5771.
  • BCHM 5776 - Scientific Ethics and Responsible Conduct in Research
    Primary Instructor - Fall 2019 / Fall 2020
    Lect. Advanced discussion of topics in scientific ethics, including requirements for responsible conduct of research, case histories of fraud, research misconduct, ethical misconduct and development of professional values and ethical standards. Same as MCDB 5776.
    • ... more

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