In vivo cross-linking followed by polyA enrichment to identify yeast mRNA binding proteins. Journal Article uri icon

Overview

abstract

  • mRNA binding proteins regulate gene expression by controlling the processing, localization, decay, and translation of messenger RNAs (mRNAs). To fully understand this process, it is necessary to identify the complete set of mRNA binding proteins. This work describes a method for the systematic identification of yeast mRNA binding proteins. This method applies in vivo UV cross-linking, affinity pull-down of polyA(+) mRNAs, and analysis by mass spectrometry to identify proteins that directly bind to mRNAs.

publication date

  • January 1, 2015

Full Author List

  • Mitchell SF; Parker R

Other Profiles

Additional Document Info

start page

  • 35

end page

  • 47

volume

  • 1259