Differential RNA-dependent ATPase activities of four rRNA processing yeast DEAD-box proteins. Journal Article uri icon

Overview

abstract

  • S. cerevisiae ribosome biogenesis is a highly ordered and dynamic process that involves over 100 accessory proteins, including 18 DExD/H-box proteins that act at discrete steps in the pathway. Although often termed RNA helicases, the biochemical functions of individual DExD/H-box proteins appear to vary considerably. Four DExD/H-box proteins, Dbp3p, Dbp4p, Rok1p, and Rrp3p, involved in yeast ribosome assembly were expressed in E. coli, and all were found to be active RNA-dependent ATPases with k(cat) values ranging from 13 to 170 min(-1) and K(M)(ATP) values ranging from 0.24 to 2.3 mM. All four proteins are activated by single-stranded oligonucleotides, but they require different chain lengths for maximal ATPase activity, ranging from 10 to >40 residues. None of the four proteins shows significant specificity for yeast rRNA, compared to nonspecific control RNAs since these large RNAs contain multiple binding sites that appear to be catalytically similar. This systematic comparison of four members of the DExD/H-box family demonstrates a range of biochemical properties and lays the foundation for relating the activities of proteins to their biological functions.

publication date

  • November 1, 2008

Date in CU Experts

  • March 13, 2015 12:56 PM

Full Author List

  • Garcia I; Uhlenbeck OC

author count

  • 2

citation count

  • 10

Other Profiles

International Standard Serial Number (ISSN)

  • 0006-2960

Electronic International Standard Serial Number (EISSN)

  • 1520-4995

Additional Document Info

start page

  • 12562

end page

  • 12573

volume

  • 47

issue

  • 47