Specific interaction of anticodon loop residues with yeast phenylalanyl-tRNA synthetase. Journal Article uri icon

Overview

abstract

  • Thirteen different yeast tRNAPhe variants with single nucleotide changes in positions 34-37 in the anticodon region were prepared by an enzymatic procedure described previously. Aminoacylation kinetics using purified yeast phenylalanyl-tRNA synthetase revealed that the level of aminoacylation was very different for different sequences inserted. The low level of aminoacylation was the result of a steady state between a slow forward reaction rate and spontaneous deacylation of the product. Aminoacylation kinetics performed at higher synthetase concentrations revealed that substitution at position 34 in tRNAPhe decreased the Km nearly 10-fold but only had a small effect on Vmax. Similar substitutions at positions 35, 36, and 37 had a lesser effect. These data suggest a sequence-specific contact between the anticodon of yeast tRNAPhe and the cognate synthetase.

publication date

  • August 1, 1982

has subject area

Date in CU Experts

  • March 13, 2015 12:57 PM

Full Author List

  • Bruce AG; Uhlenbeck OC

author count

  • 2

citation count

  • 28

Other Profiles

International Standard Serial Number (ISSN)

  • 0006-2960

Electronic International Standard Serial Number (EISSN)

  • 1520-4995

Additional Document Info

start page

  • 3921

end page

  • 3926

volume

  • 21

issue

  • 17