Quantitative analysis of atherosclerotic lesion composition in mice. Journal Article uri icon

Overview

abstract

  • Comparative quantitation has become an increasingly desirable tool in determining compositional differences of aortic plaque lesion in transgenically altered mice. To this end, methodology has been developed to identify lipid, cellularity, collagen, and elastin components using traditional bright-field microscopy, fluorescence, and polarized light microscopy, employing both confocal and wide-field imaging systems. Subsequent imaging processing and analysis on the digitally captured images reveals differences in compositional components as influenced by diet, age and gender. This method can be expanded to employ a rich variety of histochemical and immunohistochemical staining protocols.

publication date

  • January 1, 2006

Full Author List

  • Wadsworth MP; Sobel BE; Schneider DJ; Tra W; van Hirtum H; Taatjes DJ

Other Profiles

Additional Document Info

start page

  • 137

end page

  • 152

volume

  • 319