The Mediator complex is a multi-subunit assembly required for regulating expression of most RNA polymerase II (pol II) transcripts, which include protein-coding and most non-coding RNA genes. Mediator is generally targeted by sequence-specific, DNA-binding transcription factors (TFs) that control gene expression programs in response to developmental or environmental cues. Mediator functions by relaying signals from TFs directly to the pol II enzyme. Thus, Mediator is essential for converting biological inputs (communicated by TFs) to physiological responses (via changes in gene expression). The Taatjes lab examines the basic mechanisms that underlie Mediator function, with the goal of identifying key molecular interfaces (and/or molecular probes to target these interfaces) that contribute to its broad regulatory influence on gene expression. We are also actively studying factors that influence Mediator structure and activity, including TFs, non-coding RNAs, and the CDK8 module.
keywords
Transcription, structural biology, biophysics, cryo-electron microscopy, mass spectrometry, biochemistry and molecular biology, aging, signaling, metabolism
BCHM 2700 - Foundations of Biochemistry
Primary Instructor
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Spring 2021 / Spring 2022 / Spring 2023 / Spring 2024
Covers chemistry of aqueous solutions; energetics in biology; structure of proteins, nucleic acids, carbohydrates, and membranes; protein evolution; macromolecular interactions; enzyme kinetics, mechanism and regulation. Will be taught from a strong chemical perspective and mastery of basic concepts of general and organic chemistry will be required. Familiarity with basic concepts of molecular and cellular biology encouraged. Formerly CHEM 4700.
BCHM 4761 - Biochemistry Laboratory
Primary Instructor
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Fall 2019 / Fall 2020
Two 4-hour periods per week. Introduction to modern biochemical techniques. Topics include enzymology, spectrophotometry, electrophoresis, multi-step protein purification, recombinant DNA techniques and molecular cloning. Formerly CHEM 4761.
BCHM 6901 - Research in Biochemistry
Primary Instructor
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Fall 2019 / Spring 2020 / Fall 2020 / Spring 2021 / Fall 2021 / Spring 2022 / Fall 2022 / Fall 2023
May be repeated up to 15 total credit hours.
BCHM 6951 - Master's Thesis
Primary Instructor
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Spring 2023
Students are not admitted for the master's degree but may be transferred to the MS plan if they are unable to meet the demands of the PhD program.
CHEM 4400 - Core Concepts in Physical Chemistry for Biochemists
Primary Instructor
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Spring 2019
Introduces thermodynamics, kinetics and spectroscopy, emphasizing macromolecule and biochemical applications. Includes thermodynamics, chemical and physical equilibriums, solution chemistry, rates of chemical and biochemical reactions, chemical bonds and principles and selected examples of spectroscopies applied to biological systems. Department enforced prerequisite or corequisite: PHYS 1120 or PHYS 2020. Formerly CHEM 4411. Same as BCHM 5400 and CHEM 4511.
CHEM 5781 - Advanced General Biochemistry 2
Primary Instructor
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Spring 2018
Lect. Detailed consideration of contemporary topics in biochemistry, including protein structure (primary, secondary, tertiary, and quaternary), methods of structure determination and prediction, protein folding (kinetics, thermodynamics, denaturation, and renaturation), and protein dynamics (internal motions and methods of analysis). Formerly CHEM 5781.
CHEM 6901 - Research in Chemistry
Primary Instructor
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Spring 2018 / Fall 2018 / Spring 2019
May be repeated up to 15 total credit hours.
MCDB 4980 - Honors Research
Primary Instructor
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Fall 2019
Provides faculty-supervised research for students who have been approved by the departmental honors committee. Normally taken during the semester before completion of the honors thesis. Recommended prerequisite: MCDB 4840 or comparable research experience, and minimum GPA of 3.20.
