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Taatjes, Dylan J Professor

Positions

Research Areas research areas

Research

research overview

  • The Mediator complex is a multi-subunit assembly required for regulating expression of most RNA polymerase II (pol II) transcripts, which include protein-coding and most non-coding RNA genes. Mediator is generally targeted by sequence-specific, DNA-binding transcription factors (TFs) that control gene expression programs in response to developmental or environmental cues. Mediator functions by relaying signals from TFs directly to the pol II enzyme. Thus, Mediator is essential for converting biological inputs (communicated by TFs) to physiological responses (via changes in gene expression). The Taatjes lab examines the basic mechanisms that underlie Mediator function, with the goal of identifying key molecular interfaces (and/or molecular probes to target these interfaces) that contribute to its broad regulatory influence on gene expression. We are also actively studying factors that influence Mediator structure and activity, including TFs, non-coding RNAs, and the CDK8 module.

keywords

  • Transcription, structural biology, biophysics, cryo-electron microscopy, mass spectrometry, biochemistry and molecular biology, aging, signaling, metabolism

Publications

selected publications

Teaching

courses taught

  • BCHM 2700 - Foundations of Biochemistry
    Primary Instructor - Spring 2021
    Covers chemistry of aqueous solutions; energetics in biology; structure of proteins, nucleic acids, carbohydrates, and membranes; protein evolution; macromolecular interactions; enzyme kinetics, mechanism and regulation. Will be taught from a strong chemical perspective and mastery of basic concepts of general and organic chemistry will be required. Familiarity with basic concepts of molecular and cellular biology encouraged. Formerly CHEM 4700.
  • BCHM 4761 - Biochemistry Laboratory
    Primary Instructor - Fall 2019 / Fall 2020
    Two 4-hour periods per week. Introduction to modern biochemical techniques. Topics include enzymology, spectrophotometry, electrophoresis, multi-step protein purification, recombinant DNA techniques and molecular cloning. Formerly CHEM 4761.
  • BCHM 6901 - Research in Biochemistry
    Primary Instructor - Fall 2019 / Spring 2020 / Fall 2020 / Spring 2021 / Fall 2021
    May be repeated up to 15 total credit hours.
  • CHEM 4400 - Core Concepts in Physical Chemistry for Biochemists
    Primary Instructor - Spring 2019
  • CHEM 5781 - Advanced General Biochemistry 2
    Primary Instructor - Spring 2018
  • CHEM 6901 - Research in Chemistry
    Primary Instructor - Spring 2018 / Fall 2018 / Spring 2019
    May be repeated up to 15 total credit hours.
  • MCDB 4980 - Honors Research
    Primary Instructor - Fall 2019
    Provides faculty-supervised research for students who have been approved by the departmental honors committee. Normally taken during the semester before completion of the honors thesis. Recommended prerequisite: MCDB 4840 or comparable research experience, and minimum GPA of 3.20.

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