Two-dimensional gel electrophoresis for the identification of signaling targets. Journal Article uri icon

Overview

abstract

  • Two-dimensional electrophoresis (2-DE) is a powerful technique to differentially display patterns of protein expression and posttranslational modifications, providing a good strategy to monitor molecular responses induced by the activation or inactivation of specific signaling pathways. In this chapter, optimized protocols for 2-DE using extracts from tissue culture are provided. Protocols for in-gel digestion of gel-resolved proteins, which allow protein identification by mass spectrometry are also discussed.

publication date

  • January 1, 2004

Full Author List

  • Kabuyama Y; Polvinen KK; Resing KA; Ahn NG

Other Profiles

Additional Document Info

start page

  • 37

end page

  • 49

volume

  • 284