Two-dimensional gel electrophoresis for the identification of signaling targets. Journal Article uri icon

Overview

abstract

  • Two-dimensional electrophoresis (2-DE) is a powerful technique to differentially display patterns of protein expression and posttranslational modifications, providing a good strategy to monitor molecular responses induced by the activation or inactivation of specific signaling pathways. In this chapter, optimized protocols for 2-DE using extracts from tissue culture are provided. Protocols for in-gel digestion of gel-resolved proteins, which allow protein identification by mass spectrometry are also discussed.

publication date

  • January 1, 2004

has subject area

Date in CU Experts

  • September 6, 2013 4:27 AM

Full Author List

  • Kabuyama Y; Polvinen KK; Resing KA; Ahn NG

author count

  • 4

citation count

  • 1

Other Profiles

International Standard Serial Number (ISSN)

  • 1064-3745

Electronic International Standard Serial Number (EISSN)

  • 1940-6029

Additional Document Info

start page

  • 37

end page

  • 49

volume

  • 284