Isolation, sequencing and overexpression of the gene encoding the theta subunit of DNA polymerase III holoenzyme. Journal Article uri icon

Overview

abstract

  • The gene encoding the theta subunit of DNA polymerase III holoenzyme, designated holE, was isolated using a strategy in which peptide sequence was used to derive a DNA hybridization probe. Sequencing of the gene, which maps to 41.43 centisomes of the chromosome, revealed a 76-codon open reading frame predicted to produce a protein of 8,846 Da. When placed in a tac promoter expression vector, the open reading frame directed expression of a protein, that comigrated with authentic theta subunit from purified holoenzyme, to 6% of total soluble protein.

publication date

  • July 1, 1993

has subject area

Date in CU Experts

  • October 1, 2013 12:09 PM

Full Author List

  • Carter JR; Franden MA; Aebersold R; Kim DR; McHenry CS

author count

  • 5

citation count

  • 9

Other Profiles

International Standard Serial Number (ISSN)

  • 0305-1048

Electronic International Standard Serial Number (EISSN)

  • 1362-4962

Additional Document Info

start page

  • 3281

end page

  • 3286

volume

  • 21

issue

  • 14