• Contact Info
Publications in VIVO
 

Perkins, Thomas T. Professor Adjunct, Professor Adjoint

Positions

Research Areas research areas

Research

research overview

  • Single molecule studies are increasingly important in biophysics. The Perkins group focuses on developing high precision single-molecule techniques and applying them to answer a range of biological questions, such as: how motor proteins transduce chemical energy into physical motion? And, how does the structure and dynamics of membrane proteins affect their functions? We developed a optical-trapping assay with Ångström-scale stability and resolution in 3D. We are applying these techniques to study DNA-based molecular motors, the folding/unfolding kinetics of RNA structures, and DNA overstretching. We also developed focused-ion beam modified cantilevers for dramatically improved performance of atomic force microscopy and are currently pursuing studies of diverse biological systems (globular proteins, protein-ligand bonds, membrane proteins, structured RNA). We are also leveraging our advances in AFM instrumentation to achieve high-resolution imaging of DNA and protein-DNA complexes.

keywords

  • biophysics, single molecule, membrane protein, DNA, RNA, molecule motors,atomic force microscopy, optical trapping,nanotechnology, nanoscience

Publications

selected publications

Background

International Activities

global connections related to teaching and scholarly work (in recent years)

Other Profiles