Different aa-tRNAs are selected uniformly on the ribosome. Journal Article uri icon

Overview

abstract

  • Ten E. coli aminoacyl-tRNAs (aa-tRNAs) were assessed for their ability to decode cognate codons on E. coli ribosomes by using three assays that evaluate the key steps in the decoding pathway. Despite a wide variety of structural features, each aa-tRNA exhibited similar kinetic and thermodynamic properties in each assay. This surprising kinetic and thermodynamic uniformity is likely to reflect the importance of ribosome conformational changes in defining the rates and affinities of the decoding process as well as the evolutionary "tuning" of each aa-tRNA sequence to modify their individual interactions with the ribosome at each step.

publication date

  • July 1, 2008

has subject area

Date in CU Experts

  • March 13, 2015 12:56 PM

Full Author List

  • Ledoux S; Uhlenbeck OC

author count

  • 2

citation count

  • 41

Other Profiles

International Standard Serial Number (ISSN)

  • 1097-2765

Electronic International Standard Serial Number (EISSN)

  • 1097-4164

Additional Document Info

start page

  • 114

end page

  • 123

volume

  • 31

issue

  • 1