Sequence-specific interaction of R17 coat protein with its ribonucleic acid binding site. Journal Article uri icon

Overview

abstract

  • The interaction between phage R17 coat protein and its RNA binding site for translational repression was studied as an example of a sequence-specific RNA--protein interaction. Nuclease protection and selection experiments define the binding site to about 20 contiguous nucleotides which form a hairpin. A nitrocellulose filter retention assay is used to show that the binding between the coat protein and a synthetic 21-nucleotide RNA fragment conforms to a simple bimolecular reaction. Unit stoichiometry and a Kd of about 1 nM are obtained at 2 degrees C in buffer containing 0.19 M salt. The interaction is highly sequence specific since a variety of RNAs failed to compete with the 21-nucleotide fragment for coat protein binding.

publication date

  • May 1, 1983

has subject area

Date in CU Experts

  • March 13, 2015 12:57 PM

Full Author List

  • Carey J; Cameron V; de Haseth PL; Uhlenbeck OC

author count

  • 4

citation count

  • 97

Other Profiles

International Standard Serial Number (ISSN)

  • 0006-2960

Electronic International Standard Serial Number (EISSN)

  • 1520-4995

Additional Document Info

start page

  • 2601

end page

  • 2610

volume

  • 22

issue

  • 11