Enzymatic synthesis of a 21-nucleotide coat protein binding fragment of R17 ribonucleic acid. Journal Article uri icon

Overview

abstract

  • An oligoribonucleotide with a sequence identical with the bacteriophage R17 replicase initiator region has been synthesized. The sequence also encompasses the binding domain of R17 coat protein, which is known to act as a translational repressor at this site. The 21-nucleotide fragment was synthesized entirely by enzymatic methods, T4 RNA ligase being used to join shorter oligomers. The resulting fragment has a secondary structure with the expected thermal stability. Since the synthetic fragment binds R17 coat protein with the same affinity as a 59-nucleotide fragment isolated from R17 RNA, we conclude that it has full biological activity.

publication date

  • September 1, 1982

Date in CU Experts

  • March 13, 2015 12:57 PM

Full Author List

  • Krug M; de Haseth PL; Uhlenbeck OC

author count

  • 3

citation count

  • 11

Other Profiles

International Standard Serial Number (ISSN)

  • 0006-2960

Electronic International Standard Serial Number (EISSN)

  • 1520-4995

Additional Document Info

start page

  • 4713

end page

  • 4720

volume

  • 21

issue

  • 19